1 1. Introduction to Biological Chemistry II 2 2. Protein Synthesis 1 3 R1. Determining, Analyzing, and Understanding Protein Structures 4 3. Protein Synthesis 2 5 4. Protein Synthesis 3 6 5. Protein Synthesis 4 7 R2. Pre-Steady State and Steady-State Kinetic Methods Applied to Translation 8 6. Protein Synthesis 5 9 7. Protein Synthesis 6 10 8. Protein Folding 1 11 R3. Pre-Steady State and Steady-State Kinetic Methods Applied to Translation 12 9. Protein Folding 2 13 10. Protein Folding 3 14 11. Protein Folding 4 15 R4. Purification of Native and Mutant Ribosomes, Protein Purification 16 12. Protein Degradation 1 17 13. Protein Degradation 2 18 14. Protein Degradation 3 19 R5. Overview of Cross-Linking, Including Photo-Reactive Cross-Linking Methods 20 15. PK and NRP Synthases 1 21 16. PK and NRP Synthases 2 22 R6. Macromolecular Electron Microscopy Applied to Fatty Acid Synthase 23 17. PK and NRP Synthases 3 24 18. PK and NRP Synthases 4 25 R7. Application of Single Molecule Methods 26 19. Cholesterol Biosynthesis 1 27 20. Cholesterol Biosynthesis 2 28 21. Cholesterol Biosynthesis 3 & Cholesterol Homeostasis 1 29 R8. Application of CRISPR to Study Cholesterol Regulation 30 22. Cholesterol Homeostasis 2 31 23. Cholesterol Homeostasis 3 32 24. Cholesterol Homeostasis 4 33 R9. Cholesterol Homeostasis and Sensing 34 25. Cholesterol Homeostasis 5 & Metal Ion Homeostasis 1 35 26. Metal Ion Homeostasis 2 36 27. Metal Ion Homeostasis 3 37 R10. Metal-Binding Studies and Dissociation Constant Determination 38 28. Metal Ion Homeostasis 4 39 29. Metal Ion Homeostasis 5 40 R11. Mass Spectrometry 41 30. Metal Ion Homeostasis 6 42 31. Metal Ion Homeostasis 7 & Reactive Oxygen Species 1 43 R12. Mass Spectrometry of the Cysteine Proteome 44 32. Reactive Oxygen Species 2 45 33. Reactive Oxygen Species 3 46 34. Reactive Oxygen Species 4 & Nucleotide Metabolism 1 47 R13. Fluorescence Methods 48 35. Nucleotide Metabolism 2 49 36. Nucleotide Metabolism 3