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Uedu Open / Biological Chemistry II
5.08J

Biological Chemistry II

Prof. Joanne Stubbe, Prof. Elizabeth Nolan | Spring 2016
Science & Math Biology Chemistry Biochemistry Science Structural Biology
前往原始課程
CC BY-NC-SA 4.0
課程簡介
This course is an advanced treatment of biochemical mechanisms that underlie biological processes. Topics include macromolecular machines such as the ribosome, the proteasome, fatty acid synthases as a paradigm for polyketide synthases and non-ribosomal polypeptide synthases, and polymerases. Emphasis will be given to the experimental methods used to unravel how these processes fit into the cellular context as well as the coordinated regulation of these processes.
課程資訊
來源MIT 開放式課程
科系Biology
語言English
影片數49
課程影片 (49)
1
1. Introduction to Biological Chemistry II
1. Introduction to Biological Chemistry II
2
2. Protein Synthesis 1
2. Protein Synthesis 1
3
R1. Determining, Analyzing, and Understanding Protein Structures
R1. Determining, Analyzing, and Understanding Protein Structures
4
3. Protein Synthesis 2
3. Protein Synthesis 2
5
4. Protein Synthesis 3
4. Protein Synthesis 3
6
5. Protein Synthesis 4
5. Protein Synthesis 4
7
R2. Pre-Steady State and Steady-State Kinetic Methods Applied to Translation
R2. Pre-Steady State and Steady-State Kinetic Methods Applied to Translation
8
6. Protein Synthesis 5
6. Protein Synthesis 5
9
7. Protein Synthesis 6
7. Protein Synthesis 6
10
8. Protein Folding 1
8. Protein Folding 1
11
R3. Pre-Steady State and Steady-State Kinetic Methods Applied to Translation
R3. Pre-Steady State and Steady-State Kinetic Methods Applied to Translation
12
9. Protein Folding 2
9. Protein Folding 2
13
10. Protein Folding 3
10. Protein Folding 3
14
11. Protein Folding 4
11. Protein Folding 4
15
R4. Purification of Native and Mutant Ribosomes, Protein Purification
R4. Purification of Native and Mutant Ribosomes, Protein Purification
16
12. Protein Degradation 1
12. Protein Degradation 1
17
13. Protein Degradation 2
13. Protein Degradation 2
18
14. Protein Degradation 3
14. Protein Degradation 3
19
R5. Overview of Cross-Linking, Including Photo-Reactive Cross-Linking Methods
R5. Overview of Cross-Linking, Including Photo-Reactive Cross-Linking Methods
20
15. PK and NRP Synthases 1
15. PK and NRP Synthases 1
21
16. PK and NRP Synthases 2
16. PK and NRP Synthases 2
22
R6. Macromolecular Electron Microscopy Applied to Fatty Acid Synthase
R6. Macromolecular Electron Microscopy Applied to Fatty Acid Synthase
23
17. PK and NRP Synthases 3
17. PK and NRP Synthases 3
24
18. PK and NRP Synthases 4
18. PK and NRP Synthases 4
25
R7. Application of Single Molecule Methods
R7. Application of Single Molecule Methods
26
19. Cholesterol Biosynthesis 1
19. Cholesterol Biosynthesis 1
27
20. Cholesterol Biosynthesis 2
20. Cholesterol Biosynthesis 2
28
21. Cholesterol Biosynthesis 3 & Cholesterol Homeostasis 1
21. Cholesterol Biosynthesis 3 & Cholesterol Homeostasis 1
29
R8. Application of CRISPR to Study Cholesterol Regulation
R8. Application of CRISPR to Study Cholesterol Regulation
30
22. Cholesterol Homeostasis 2
22. Cholesterol Homeostasis 2
31
23. Cholesterol Homeostasis 3
23. Cholesterol Homeostasis 3
32
24. Cholesterol Homeostasis 4
24. Cholesterol Homeostasis 4
33
R9. Cholesterol Homeostasis and Sensing
R9. Cholesterol Homeostasis and Sensing
34
25. Cholesterol Homeostasis 5 & Metal Ion Homeostasis 1
25. Cholesterol Homeostasis 5 & Metal Ion Homeostasis 1
35
26. Metal Ion Homeostasis 2
26. Metal Ion Homeostasis 2
36
27. Metal Ion Homeostasis 3
27. Metal Ion Homeostasis 3
37
R10. Metal-Binding Studies and Dissociation Constant Determination
R10. Metal-Binding Studies and Dissociation Constant Determination
38
28. Metal Ion Homeostasis 4
28. Metal Ion Homeostasis 4
39
29. Metal Ion Homeostasis 5
29. Metal Ion Homeostasis 5
40
R11. Mass Spectrometry
R11. Mass Spectrometry
41
30. Metal Ion Homeostasis 6
30. Metal Ion Homeostasis 6
42
31. Metal Ion Homeostasis 7 & Reactive Oxygen Species 1
31. Metal Ion Homeostasis 7 & Reactive Oxygen Species 1
43
R12. Mass Spectrometry of the Cysteine Proteome
R12. Mass Spectrometry of the Cysteine Proteome
44
32. Reactive Oxygen Species 2
32. Reactive Oxygen Species 2
45
33. Reactive Oxygen Species 3
33. Reactive Oxygen Species 3
46
34. Reactive Oxygen Species 4 & Nucleotide Metabolism 1
34. Reactive Oxygen Species 4 & Nucleotide Metabolism 1
47
R13. Fluorescence Methods
R13. Fluorescence Methods
48
35. Nucleotide Metabolism 2
35. Nucleotide Metabolism 2
49
36. Nucleotide Metabolism 3
36. Nucleotide Metabolism 3